Effect of morphine on activity of five selected lysosomal enzymes and kidneys of mice

The experiment was carried out on 90 Swiss male mice divided into 9 groups (n=10). Over 4, 10 and 14 days mice of three control groups (I-III) were injected with 250 μl 0.9% NaCl solution daily, and those from six experimental groups (A-F) with 250 μl 0.9% NaCl solution containing 20 or 30 mg morphine hydrochloride per kg body weight. The injections were given intramuscularly once a day between 9:00-10:00 a.m. for 4, 10 and 14 days. In the lysosomal fraction of the liver and kidney the activities of acid phosphatase, lysosomal esterase, β-glucuronidase, β-galactosidase, and β-N-acetyl-hexosaminidase were estimated. Morphine increased the activity of all examined enzymes except EL, which activity was statistically proven to decrease in liver and kidneys after 10 days morphine administration in both doses.