Investigations on parainfluenza-3 virus isolated from calves in Poland. Structure of virion and inclusion bodies

The results of numerous investigations on structure and replication of the parainfluenza-3 virus (P1-3) are not always in accordance. It is true, among other things, for appearance of inclusion bodies in infected cells, what is of importance for diagnostical examinations. These bodies were not observed by Cochen et al. (4) in the amnion cells of hen or man infected with the strain of this virus isolated from men, whereas by Bögel and Klinger (11, 12) the occurrence of eosinophilic inclusions in nuclei and cytoplasm of calf kidney cells infected with the above virus have been described, while Karten and Churchill (7), Kuhn and Kita (6) and Ané (1) have found these bodies only in cytoplasm of the same cells. It follows from the investigations of Kita (8) that the parainfluenza-3 virus strains isolated from cattle in Poland are capable to induce acidophilic inclusions in cytoplasm of the cells of calf kidney tissue cultures. The reason of such divergent observations can be perceived in different properties of various cells of hosts and various P1-3 virus strains. In this connection it has been decided to investigate in the present work the structure of virions as well as the structure and location of inclusion bodies in the calf kidney cells infected with the virus.