Age as a factor influencing diversity of commensal E. coli microflora in pigs

Commensal, intestinal E.coli microflora plays a role in maintenance of intestinal balance of the host, is responsible for defending against pathogenic E.coli. This study encompasses the analysis of BOX-PCR fingerprinting patterns, phylogenetic grouping and virulence genes prevalence among commensal E.coli isolates derived from healthy pigs. Altogether, 274 unique E.coli isolates were identified, 110 from weaned piglets (Piglets I and Piglets II) and 164 from adult sows (Sows I and Sows II). BOX-PCR analysis distinguished isolates from pigs in different age and indicated that during maturation the changes in E.coli microflora occurred. Phylogenetic grouping revealed significant differences between distribution of four phylogenetic groups among isolates derived from piglets and sows. In phylogenetic structure of isolates from the piglets group B1 prevailed significantly, while among isolates derived from the sows the majority of them were classified into phylogenetic group A. The identification of 17 virulence factors in E.coli isolates derived from healthy pigs was performed. Three of 13 intestinal (escV, ehxA, estII) and four extra-intestinal virulence genes (VGs) (hlyA, fimH, papA, sfaS) were detected in the porcine isolates. The percentage of VGs positive isolates among piglets is higher than among sows, moreover, the VGs occurring in E.coli isolates from piglets revealed greater diversity than that detected among isolates from sows.