RP-HPLC-DAD finger print analysis of phenolic extracts from transgenic flax

Two flax lines overexpressing genes encoding three essential enzymes of the flavonoid synthesis pathway: chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR), were generated. Phenolic compounds were extracted from the flaxseed lines and the control sample (Linola) using 80% methanol. Then the complex of phenolic compounds present in the crude extract was hydrolysed at basic conditions. The crude and hydrolysed extracts were analysed using an RP-HPLC-DAD method. The chromatograms of the crude extracts of the control sample and transgenic lines were the same. The finger print analysis of the hydrolysed extracts shows the influence of the expression of genes encoding three above mentioned enzymes on the proportion between secoisolariciresinol diglucoside (SDG) and phenolic acids derivatives forming the phenolic complex in flaxseed.

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