The Technological Parameter Optimization of Rapid Freeze Prototyping for 3D Tissue Scaffold Fabrication

When using the Rapid freeze prototype (RFP) process to fabricate 3D tissue bone scaffold, the selecting of technological parameters directly affects lap ratio, which is the foundation of continuous fabrication and scaffold quality. Through analyzing the force of non-solidified fiber, Velocity ratio, liquid temperature, solid content, nozzle diameter and row spacing have been chosen as the influence factors and the L16 (45) orthogonal experiments have been done The experiments result is that material viscosity and row spacing is the most important factors for lap ratio influence. Optimal parameters combination has been got by range analysis. Finally, the mixed solution of gelatin-chitosan experiment with the optimal parameters has successfully produced multilayer tissue bone scaffold, including multiple scale structure.

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W artykule zaprezentowano process wytwarzania materiałów trójwymiarowych metoda gwałtownego oziębiania – RFP (rapid freeze prototype). Materiałem wyjściowym jest mieszanina żelatyny i chitozanu. Na proces tworzenia struktury ma wpływ wiele czynników, takich jak: temperatura cieczy, zawartość składnika stałego, rozmiary węzłów, średnica i odstępy między rzędami.