Simple and sensitive LC-UV method for simultaneous analysis of hydrochlorothiazide and candesartan cilexetil in pharmaceutical formulations

A simple, sensitive, and inexpensive high-performance liquid-chromatographic method has been developed for simultaneous determination of hydrochlorothiazide and candesartan cilexetil in pharmaceutical formulations. Chromatographic separation was achieved on a Phenyl-2 column with a 25:75:0.2 mixture of 0.02 M potassium dihydrogen phosphate, methanol, and triethylamine, final pH 6.0 š 0.1, as mobile phase. Detection was at 271 nm. Response was a linear function of concentration in the range 5–45 µg mL-1 for hydrochlorothiazide and 12–56 µg mL-1 for candesartan cilexetil; the correlation coefficients were 0.9993 and 0.9991, respectively. Total elution time for the two components was less than 5 min.