The influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique

The study purpose was to examine the influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique. Two methods of DNA isolation were used - the Tomson-Henry method, which provides low purified DNA, and the column method, which provides high purified DNA. By using DNA isolated with these two methods the RAPD analysis of genetic distance was executed. It was noticed, that using high purified DNA resulted in higher number of amplification’s products, and consequently in larger accuracy of analysis.