In vitro investigation of anti-inflammatory activity of propolis/saffron extract/curcumin-loaded ZIF8 nanoparticles and their potential application for treating osteoarthritis

Background: Osteoarthritis (OA) poses a significant healthcare challenge globally, necessitating the development of effective therapeutic interventions. It is crucial to develop novel drug delivery systems for OA treatment. Aims: This study explores the potential of propolis, saffron extract, and curcumin-loaded zeolitic imidazolate framework-8 (ZIF8) nanoparticles as a treatment modality for OA. The anti-inflammatory and chondroprotective properties of these natural compounds make them promising candidates for OA management. Methods: Through comprehensive in vitro investigations, including scanning electron microscopy (SEM), MTT assays, antiinflammatory assays, cell migration assays, Fourier transform infrared (FTIR) spectroscopy, and release assays, we evaluated the physicochemical and biological characteristics of propolis, saffron extract, and curcumin-loaded ZIF8 nanocarriers. Results: Our Endings demonstrate that these nanocarriers effectively encapsulated the bioactive compounds, exhibited sustained release profiles, and displayed significant anti-inflammatory properties. Notably, propolis-loaded ZIF8 nanocarriers exhibited superior anti-inflammatory activity compared to other formulations. The encapsulation of propolis, saffron extract, and curcumin within ZIF8 nanoparticles holds promise for enhancing their therapeutic efficacy and ensuring targeted delivery to affected joints in OA treatment. Conclusion: This study highlights the potential of nanotechnology-based delivery systems in harnessing the therapeutic benefits of natural compounds for OA management.