Metody in-vivo badania właściwości antyoksydacyjnych związków kompleksowych

This article describes the in-vivo methods of studying the antioxidant properties of complex compounds. The reduced glutathione (GSH) method, which uses the reactivity of the reduced form of GSH with free radicals, is among the described methods. Further the in-vivo methods are based on the use of antioxidant enzymes such as glutathione peroxidase, glutathione S-transferase, superoxide dismutase, catalase. These types of enzymes occur naturally in the human body and they are responsible for the inactivation of free radicals, e. g. superoxide dismutase catalyzes the reaction of disproportionation of superoxide anion radical to water and oxygen. The next in-vivo methods described in this article use y-glutamyl transpeptidase and glutathione reductase, which are components of the antioxidant mechanism occurring in an organism. The last method described in this work relates to the lipid peroxidation, which is determined by the concentration of dimalonic aldehyde.

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Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).