Determination of the Absolute Configuration of 2-Hydroxyglutaric Acid, 5-Oxoproline and Vigabatrin in Urine by Chiral Derivatization and 13C or 19F NMR

Procedures for enantiomeric recognition of 2-hydroxyglutaric acid and 5-oxoproline - markers ofmetabolic diseases, and of vigabatrin - an antiepileptic drug, suitable for urine samples, have been elaborated. They rely upon derivatization of the metabolites with a chiral reagent; this is made without separation of these compounds from a biological sample. The diastereotopic metabolite derivatives are then distinguished using 13C or 19F NMR spectroscopy. This procedure is not so simple as one proposed previously, but, as opposite to the latter method, it seems to be unfailing and still not too complicated.