Raw data for the article "Mitochondrial Calcium Uniporter protects hippocampal CA2 neurons from excitotoxic injury"

Individual region of the hippocampus differ significantly in their sensitivity to excitotoxicity. In this project, using organotypic slice cultures, we show that spatial distribution of Mitochondrial Calcium Uniporter (MCU) protein positively correlates with regional resistance to NMDA toxicity. Moreover, we exhibit that exposure to NMDA strongly upregulates MCU expression in CA2 region, a structure known for its high resistance to damage in different hippocampal pathologies. Finally, we observe, that, unlike in other neuronal population, MCU inhibition sensitizes normally resistant CA2 neurons to excitotoxic stimuli.

The data presented here are included in a single MS Excel file with 9 spreadsheets. Spreadsheets 1-7 contain the measurements of fluorescence intensity of propidine iodide (a marker of cell damage) performed along the pyramidal layer of CA1 and CA2 regions in individual slices exposed to 25 μM NMDA and 2, 10 and 50 μM MCU-i4, a specific inhibitor of MCU. Spreadsheet 8 contains the average biological replicate values calculated for CA1 and CA2 regions based on the data presented in spreadsheets 1-7 and used to generate Fig. 4B of the manuscript and spreadsheet 9 contains the average biological replicate values calculated for CA2/CA1 ratios based on the data presented in spreadsheets 1-7 and used to generate Fig. 4C of the manuscript.