Data related to the work entitled Spectroscopic Properties and Biological Activity of Fluphenazine Conjugates with Gold Nanoparticles

The objective of this study was to develop a method for preparing stable fluphenazine (FPZ) conjugates with gold nanoparticles (AuNPs) and to explore their physicochemical and biological characteristics. The FPZ-AuNP conjugates were synthesized through a ligand exchange reaction on the surface of AuNPs, with an average diameter of 17 ± 5 nm. Electrokinetic analyses showed that the zeta potential of the FPZ-AuNPs was influenced by both their chemical composition and the pH of the surrounding medium. The conjugates exhibited an isoelectric point, attributed to the acid–base behavior of FPZ. To determine the molecular orientation of FPZ upon adsorption, surface-enhanced Raman spectroscopy (SERS) was employed, supported by density functional theory (DFT) calculations. Biological evaluation using human neuroblastoma SH-SY5Y cells demonstrated that FPZ-AuNP conjugates significantly decreased cell viability compared to either free FPZ or citrate-stabilized AuNPs alone. The observed cytotoxic effect was dose-dependent, increasing with higher concentrations of the FPZ-AuNP conjugates.