THE PREVENTIVE EFFECTS OF RUTIN ON IMMOBILIZATION STRESS-INDUCED CARDIAC DAMAGE IN RATS

Background: The previous studies formed the role of oxidants and proinflammatory cytokines in the pathogenesis of acute psychological stress-related cardiac damage leading to mortality and other complications. The aim of the study is to examine the protective effect of rutin against stress-induced cardiac damage. In the literature, no studies have been found analyzing the effects of rutin in acute stress related oxidative damage induced by immobilization method in rats.Material and Methods: Rutin was administered orally to rutin + stress applied (RSG) group albino rats at a dose of 50 mg/kg. For healthy control (HG) and stress applied to control (SAG) groups, distilled water as a solvent was orally administered at the same volume (0.5 ml). One hour after rutin and distilled water applications, all animals except for HG brought into supine position and their legs and arms were ligated and kept in the same position for 24 hours.Results: Oxidant, cytokine and cardiac biomarker levels in blood serum and heart tissues of SAG animals were found to be significantly higher and total glutathione was lower than RSG and HG groups. Histopathologically dilated conjugated blood vessels and myocardial destruction, hemorrhage, edema, and polymorphonuclear leucocyte infiltration were observed in the SAG heart tissues. On the other hand, the histological heart tissue results of the RSG group was found to be similar compare to healthy tissue except for the slightly dilated conjugated blood vessel. Conclusion: These results indicate that the rutin may be useful in the treatment of stress-related oxidative cardiac damage