Wpływ doustnego podania glinu na poziom wskaźników hematologicznych i biochemicznych krwi oraz jego zawartość w wybranych strukturach ośrodkowego układu nerwowego psów

The objective of the study was to demonstrate the effect of oral administration of aluminum on the level of hematological and biochemical blood indicators, clinical symptoms and its contents in selected CNS structures in dogs. The study was conducted in two stages. The dogs were assigned to two groups. The experimental group was composed of 7 hybrid dogs (aged 12-17 years, 5 males and 2 females) which received 10 mg/kg aluminum sulphate orally over a period of 6 weeks. The control group was composed of 7 hybrid dogs (aged 13-20 years, 3 males and 4 females) which did not receive the preparation. 1 ml blood samples for hematological tests were collected from the cephalic vein (vena cephalica) to EDTA test tubes. The number of erythrocytes (RBC), hemoglobin concentration (Hb), hematocrit (Ht), the number of leukocytes and platelets were determined. The hematological test was conducted with the automatic analyzer unit by ABX Diagnostic. 5 ml blood samples were collected for the biochemical tests into test tubes with silicone spheres. Serum was obtained from full blood for 5 min. at 1500 × g. Serum was used to assess the activity of alanine aminotransferase (ALAT/GPT), aspartate aminotransferase (AspAT/GOT), alkaline phosphatise (ALP/AP/FA), level of calcium (with the POINTE 180 biochemical analyzer unit), magnesium, organic phosphorus (with the EPOLL 11 analyzer unit), sodium, potassium, and chlorine (with the CIBA CORNING 644 analyzer unit). After 42 days of administration of aluminum, an insignificant decrease in all morphological blood parameters was recorded in the experimental group, with a statistically significant decrease found in the concentrations of calcium and potassium in blood serum between the first day of the study and the 42nd day. The level of calcium in the experimental group was reduced. No statistically significant differences were found in the concentrations of sodium, magnesium, phosphorus and chlorine in the experimental group. However, a statistically significant increase was recorded in the content of aluminum in the cerebral cortex, hippocampus and cerebellum cortex in the experimental group.