Dzialanie kadmu, selenu i obu tych pierwiastkow lacznie na synteze DNA, RNA i bialek w komorkach jelita cienkiego myszy

Przeprowadzone badania dotyczyły zmian w syntezie DNA, RNA i białek w komórkach jelita cienkiego myszy w warunkach długotrwałej, trzymiesięcznej intoksykacji kadmem i selenem oraz ustalenia, czy selen modyfikuje zmiany wywołane działaniem kadmu.

Changes of DNA, RNA and protein synthesis in small intestine cells of white Balb mice exposed for 3 months to cadmium and selenium were studied. The animals received per os cadmium (50 ppm), selenium (0,4 mg/kg b.w.), or both elements in doses as specified. The control and test animals were injected intraperitoneally a single dose (1 µСі/g b.w.) of the radioactive precursor: 63H-timidine (DNA precursor), 5,63H-uridine (RNA precursor) and L-3H-alanine (protein precursor). Cadmium caused a decrease in the intensity of DNA synthesis, and a slight increase in the intensity of RNA and protein synthesis in the small intestine cells. Three-month exposure to selenium at the threshold dose reduced the intensity of nucleic acid and protein synthesis in the cells. In that case, selenium showed a stronger toxic effect on the cellular metabolism than cadmium. However, combined exposure to cadmium and selenium increased the intensity of small intestine cell RNA synthesis. Combined cadmium/selenium exposure increased protein synthesis as compared with the animals receiving selenium only to the level similar to that observed in the controls.