Exopolysaccharide production by Bacillus strains colonizing packaging foils

The influence of the chemical composition of medium, availability of glucose and pH on the production of exopolysaccharides (EPS) by different Bacillus strains were investigated. Bacillus strains were isolated from the surface of polyethylene foils modified with mineral compounds after their biodégradation in compost soil. Moreover, the effect of EPS production on bacterial adhesion onto the surface of the materials was examined. The enhanced synthesis of exopolysaccharides in nutrient-starved conditions was revealed. The most effective synthesis of polymers was observed during the logarithmic phase of culture growth. The increased amount of EPS facilitated bacterial adhesion to material surfaces. It was determined that the biofilm on the material surface positively affects its biodégradation. Based on the results, we conclude that the biodégradation of polymers may be accelerated in low-nutrient environment.