Wystepowanie beta-laktamaz typu ESBL I AmpC u paleczek gram-ujemnych izolowanych z zywnosci

Oznaczono wytwarzanie ß-laktamaz u 739 szczepów pałeczek Gram-ujemnych wyizolowanych z żywności stosując metodę trzech krążków. Wykryto jeden szczep ESBL należący do gatunku Stenotrophomonas maltophilia i 14 szczepów produkujących ß-laktamazy AmpC należących do rodzajów Enterobacter, Hafnia, Morganella, Citrobacter, Cedecea, Acinetobacter.

The aim of the study was to isolate extended spectrum ß-laktamases (ESBL) and chromosomal ß-laktamases AmpC producing strains from food products. A total of 739 Gram-negative bacteria were tested with double disc diffusion method using cefotaxim, ceftasidim and amoxycillin with clavulanic acid. One strain producing ESBL and belonging to Stenotrophomonas maltophilia was detected from the ice cream (0.14% of all strains). From different food products a total of 14 microorganisms (1.9%) having AmpC enzymes have been isolated. They belonged to Enterobacter spp, Hafnia spp, Morganella spp, Citrobacter, Acinetobacter, and Cedecea. Appearance of strains producing ß-laktamases ESBL and AmpC among microorganism isolated from food make it necessary to monitor enzymes activity during routine microbiological control of foods.