The 45 kDa and 27 kDa yeasts protein kinases are notimmunologically related

Two yeast casein kinase type-I sp«cies of 45 kDa and 27 kDa (CK1) were purified to apparent homogeneity and used for investigation of their immunological affinity. Antiscra against the two kinases were isolated; the antibody agaiast the 45 kDa kinase did not react with the 27 kDa enzyme. The 27 kDa casein kinase was recognized only by its own antibody. The obtained data strongly suggest that the low molecular mass CK-1 is not a proteolytic product of the 45 kDa kinase species.