Wystepowanie beta-laktamaz typu ESBL i IBL u paleczek Pseudomonas aeruginosa

Aktywność enzymów o rozszerzonym spektrum działania (ESBL) wykrywano metodą dwóch krążków wg Jarliera i wsp. (8). Indukowane ß-laktamazy (IBL) wykrywano testem dwóch krążków wg Sandersów (15). Wśród pałeczek Pseudomonas aeruginosa znaleziono 8 (18,6%) szczepów produkujących ß-laktamazy o rozszerzonym spektrum działania (ESBL) i 31 (72%) szczepów wytwarzających ß-laktamazy indukowane (IBL).

The aim of the study was to determine extended spectrum ß-lactamases (ESBL) and inducible ß-lactamases (IBL) among Pseudomonas aeruginosa strains. A total of 43 strains isolated humans (6), hospital sink (1), fish (15), cattle (5), swine (5), dog (1), redder (1), fur animals (9) were studied. ESBL-producing strains were detected with double disc diffusion test according to Jarlier et al. (8). Clavulonate and tazobactam were used as the inhibitors of ESBL. Inducible ß-lactamases were determined using double disc method according to Sanders (15). Cefoxitin was the inductor of these ß-lactamases. The susceptibility study was carried out using the disc diffusion method according to NCCLS standards. A total of 8 ESBL (18,6 % of all strains) and 31 (72%) IBL producing strains were detected. The obtained results indicate the necessity of monitoring of ESBL- and IBL-producing strains of Pseudomonas aeruginosa.