Application of nested PCR for the detection of canine parvovirus in faeces

In our studies two primer pairs within the VP2 protein gene of canine parvovirus were chosen on the base of the sequence of Polish isolates. The sensitivity of detection of canine parvovirus in stool samples by Nested PCR was increased 60% in comparison with the standard PCR method and it was 30% higher than the virus isolation in tissue culture. A simple procedure of sample preparation with the use of Chelating resin was used and it made the Nested PCR easily applicable in rapid confirmation of the diagnosis of parvovirus infection.