Zmiany ilosciowe populacji limfocytow CD3 plus i CD45RA plus we krwi szczurow poddanych dzialaniu wolnozmiennego pola elektromagnetycznego

It was reported that an extremely low frequency (ELF) electromagnetic field causes biological effects in vitro and in vivo. The major functions of the immune system are to develop the concept of “self” and eliminate what is “non-self”. In our preliminary study we proved that an extremely low frequency (ELF) magnetic field increased IgG concentration in the serum of rats. The study was carried out on male Wistar rats. The rats were exposed during 3 or 6 days (8 minute a day) to an ELF magnetic field of a complex shape generated by a device used in medicine. Five groups were used in this experiment: Group I - control group, absence of ELF magnetic field, Group II - exposure during 3 days (B = 0.06 mT), Group III - exposure during 3 days (B = 0.14 mT), Group IV - exposure during 6 days (B = 0.06 mT), Group V - exposure during 6 days (B = 0.14 mT). The animals were sacrificed for the experiment on the 2nd day after exposure to the ELF magnetic field. The leukocytosis and lymphocytes subpopulations (CD3+ and CD45RA+) in the blood of rats were assayed. Our results did not show significant changes of leukocytosis in groups II, III and IV. In rats of group V the leukocytosis decreased. In all groups we observed a decrease of CD3+ lymphocytes during the experiment. ELF magnetic field (B = 0.14 mT) induced a decrease of CD45RA+ lymphocytes in rats of groups III and V.