Biochemical changes in seminal plasma after endotoxin injection in stallions

The study was made on 17 stallions of Polish Primitive Horses divided into four groups: I - control group, II - subject to LPS Escherichia coli injection in the dose of 0.05 μg/kg b.w., III - LPS in the dose of 0.1 μg/kg b.w. and IV - LPS in the dose of 0.3 μg/kg b.w. (n=5). The study was meant to determine the concentration of total protein (TPR), albumin (ALB) and aspartate aminotransferase (AspAT) in seminal plasma. Changes in the levels of all the above biochemical components in seminal plasma were noticed in stallions under endotoxin influence, especially after administration of the highest LPS dose. A statistically significant increase was noticed in TPR concentration (maximum in the 8th week), ALB concentration (maximum in the 24th hour). Changes in AspAT activity was also found with a maximum increase in the 6th week after LPS E. coli administration. The results of these studies show that endotoxemia induces changes in the biochemical properties of the seminal plasma in stallions, especially with reference to enzyme activity.