Ocena subpopulacji limfocytow oraz wybranych cytokin u krolikow z przewlekla trichofitoza

The aim of the study was to carry out a cytometric analysis of a lymphocyte subpopulation with markers CD3, CD4, CD8, CD19 and CD25 and to ascertain the serum level of TNF-α and IL-10 in the peripheral blood of rabbits with chronic trichophytosis, caused by Trichophyton mentagrophytes. The suppression of cellular immune responses in trichophytic rabbits was characterized by an increase in the percentage of suppressor lymphocytes TCD8⁺ and a decrease in the proportion of TCD4⁺/TCD8⁺. The immunological profile of rabbits with chronic trichophytosis was characterized by an increased level of Th2 and, hence, by an increased production of IL-10. The high concentrations of IL-10 most probably generated anergic lymphocytes TCD4⁺ and TCD8⁺ which, in turn, induced antigenic specific immunosuppression. This is due to the key role that this cytokine plays in regulating immune responses aimed at restricting inflammation and increasing humoral immune responses while simultaneously blocking Th1 type cytokines.