Effect of selected lipid substrates on the process of biosynthesis of surface-active compounds by the Candida bombicola yeasts

The performed investigations were concerned with the possibility of utilisation of the yeasts ATCC 22214 Candida bombicola for the biosynthesis of surface active substances on substrates containing oleic acid, sunflower oil and post-deodorising condensate as carbon sources. Glucose was the source of hydrophilic carbon in the medium, whereas nitrogen was supplied by the yeast extract. The highest yields of biosurfactants (124.5 g·dm-3 - oleic acid, 116 g·dm-3 - sunflower oil and 118.5 g·dm-3 - post-deodorising condensate) were achieved using the medium in which 3 g of glucose and 0.05 g of yeast extract and 1 g of the fat substrate. The obtained biosurfactants, depending on the fat substrate applied in the culture medium, differed with respect to their appearance. The obtained preparations reduced the surface tension of water from 70 mN/m to approximately 32 mN/m.