Characterization of the first Polish isolate of Neospora caninum from cattle

Neospora caninum was isolated from the brain of an apparently healthy calf born to a seropositive cow. The calf was killed 12 hrs after birth and homogenate of its brain was inoculated into Vero cells. Neospora-like tachyzoites were detected 66 days later. The identity of the parasite was confirmed by polymerase chain reaction amplification of N. canium-specific fragments using Np21 and Np6 primers. This first Polish isolate of N. caninum was designated NC-PolB1.