Charakterystyka molekularna szczepow Staphylococcus aureus i Staphylococcus epidermidis wyizolowanych z materialu klinicznego

Scharakteryzowano szczepy Staphylococcus aureus i Staphylococcus epidermidis, wyizolowane z ran i tkanek głębokich oraz z powierzchni skóry, pobranych w celu poszukiwania obecności genów dla czynników wirulencji. Określono genetyczne zróżnicowanie badanych szczepów uzyskane metodą reakcji PCR ze specyficznymi starterami.

The aim of study was the molecular characteristic of S. aureus and S. epidermidis isolates obtained from skin surface, wounds, deep tissues of hospitalized patients and from skin surface of non-hospitalized patients. Genes encoding virulence factors were examined using PCR reaction and specific primers. Genes encoding adhesins fnbA and cna and gene eta for epidermolytic toxin were mostly present in S. aureus isolates coming from wounds and deep tissues compared to these from skin surface. Gene atlE encoding autolysin of S. epidermidis was detected in all studied isolates, whereas gene icaAB was present in almost all isolates. Comparison of results obtained by PCR and conventional method of the resistance to methicillin estimation showed discrepances suggesting the need for using of both methods in some clinically difficult cases of S. aureus infection.