Dzialanie in vitro bakterii z rodzaju Lactobacillus izolowanych z pochwy na grzyby wywolujace kandydoze sromu i pochwy

Bakterie Lactobacillus delbrueckii, występujące w mikroflorze pochwy wspólnie z gatunkami z grupy Lactobacillus acidophilus, hamują wzrost Candida albicans w czasie 90 minut, zaś bakterie z gatunku Lactobacillus plantarum - po 24 godzinach. Wczesne działanie Lactobacillus można przypisać nadtlenkowi wodoru, zaś późne - innym metabolitom. W warunkach naturalnych bakterie z rodzaju Lactobacillus prawdopodobnie współdziałają ze sobą przeciwko C. albicans, wykorzystując kilka metabolitów.

Growing frequency of therapeutical failures of vulvovaginal candidiasis, resulting from resistance of certain species of Candida to imidazole agents, raises interest in the use of probiotics from Lactobacillus genera as prophylaxis. Unfortunately, little is known about inhibitory mechanisms of Lactobacillus on Candida. The aim of this study was to compare the activity of selected Lactobacillus species, representing the physiological vaginal flora, against Candida as well as investigation whether their inhibitory activity against Candida is related strictly to hydrogen peroxide and lactic acid production. 125 strains from vaginal smears of healthy women were classified by making use of phenotypic and genotypie methods. The majority of strains belonged toL. acidophilus: L. acidophilus sensu stricto, L. crispatus, L. gasseri and L. johnsonii as well as L. fermentům and L. plantarum species. Culture supernatants of selected 25 strains representing the isolated species were examined for their inhibitory activity against the growth of Candida albicans and C. glabrata. The results showed that the strongest and the fastest activity against C. albicans was demonstrated by L. delbrueckii strains, producing the largest quantities of hydrogen peroxide. On the other hand, extended activity, demonstrable after 24 hours, was shown by non-H2O2 producing L. plantarum supernatants. Growth of C. glabrata was not inhibited by any of the examined strains of Lactobacillus. Comparison of activity of live active cultures of Lactobacillus strains and their mixtures with this of pure H2O2 and lactic acid has shown that pure chemical compounds were less active than the cultures. This suggests that mixtures of Lactobacillus strains are in cooperation with each other using many different metabolites.