Charakterystyka szczepow Staphylococcus aureus izolowanych z probek zywnosci

Scharakteryzowano szczepy Staphylococcus aureus izolowane z próbek żywności, pobranych w związku z poszukiwaniem czynnika etiologicznego w ognisku zatrucia pokarmowego. Na podstawie analizy restrykcyjnych wzorów chromosomalnego DNA, uzyskanych metodą PFGE, określono genetyczne podobieństwo badanych szczepów S. aureus oraz oceniono ich zdolność do wytwarzania enterotoksyny przy użyciu testu SET-RPLA.

The study was carried out of 22 isolates of S. aureus isolated from 7 different incriminated food's samples from foodborne-disease outbreaks. The possibility of these isolates to producing of enterotoxins by commercial test SET-RPLA (Oxoid) was tested. The genotyping of these isolates was done by pulse-filed gel electrophoresis, acc. to Pfaller in own modification. On the basis of the DNA restriction patterns of the 22 isolates - 5 strains were singled out, one of these strains - strain V (isolat nr 7) was not relationship to others. It was found that this strain V was one enterotoxin produced. Additionaly, all tested strains, in spite of the strain nr V, were isolated from the 2 or 3 samples of different kinds of foods. In the present study it has been shown too, that several similar colonies should be isolated for farther studies to assess microbiological contamination of the food products properly.