In vitro storage Polypodium vulgare L. rhizome shoot tips using ABA treatment before dehydration - encapsulation technique

An efficient protocol for storage of Polypodium vulgare rhizome shoot tips was developed. The best result was observed when the rhizomes were pretreated with 2 mg l-1 abscisic acid/24 h and dehydrated 10 h in hypertonic solution of 20% mannitol before shoot tip isolation. Those explants were encapsulated and stored for 2 months. Plantlets of good quality regenerated after the capsules were transferred to Murashige Fern Multiplication Medium.