Effect of neuraminidase on adherence of Pseudomonas aeruginosa to human buccal epithelial cells. Inhibition of adhesion by monosaccharides

The aim of this study was to evaluate the action of Clostridium perfringens neuraminidase on the adherence of 28 strains of Pseudomonas aeruginosa which were isolated from humans, different animals and environment to human buccal epithelial cells (BECs). Two reference strains - NCTC 6749 and ATCC 27853 were also examined. Incubation of cells with the enzyme significantly increased bacterial adherence (a mean number of bacteria adhering to cells amounted 19.62 ±9.20, for controls - 7.54 ±5.86). The reference strains of Pseudomonas aeruginosa showed the following adherence: NCTC 6749-43.04 (control 20.83) and ATCC 27853-22.21 (control 5.51). This study demonstrates that asialogangliosides function as receptors on buccal epithelial cells for P. aeruginosa strains. Monosaccharides inhibition studies showed an inhibition of adhesion of P. aeruginosa (two reference strains - NCTC 6749 and ATCC 27853, two hospital strains - 80/85 and 351) to normal BECs in the presence of N-acetylneuraminic acid and N-acetylgalactosamine. D-galactose is the best inhibitor of bacterial adhesion to neuraminized BECs. All monosaccharides used had a significant effect on P. aeruginosa adherence to trypsinized BECs. These data suggest a difference in the receptors on the three types of BECs.