New inducers for cellulases production by Trichoderma reesei M-7

The aim of work was to examine the influence of lactobionic acid, gluconic acid, delta-gluconolactone, lactulose and sorbitol on the production of cellulases by mutant strain Trichoderma reesei M-7 during batch and continuous cultivation. Positive results were achieved in the presence of lactobionic acid and lactulose as inducers. In both cases the highest inductive effect of cellulase production was observed in the presence of 1% mixtures of above mentioned substrates at the ratio 1:1 (0.5% : 0.5%) with lactose. (about 20% higher than that observed in the presence of 1% of lactose) The induction mechanism of lactobionic acid and lactulose may be attributed by slow rate of their utilisation (slower than lactose). Explanation of the inductive mechanism of lactose, lactobionic acid and lactulose on cellulases production by Trichoderma reesei demands further investigations among other with using a purified beta-galactosidase enzyme.