The isolation and identification of prostaglandins of Trichostrongylus colubriformis and Haemonchus contortus

Prostaglandins (PGs) have been implicated as precursors of biological processes, many of which are functional in parasite-host interactions. The aim of the study was the isolation and examination of PGs in extracts from adults, larvae and excretory/secretory products (E/S) of Trichostrongylus colubriformis and Haemonchus contortus. Thin-layer chromatographic (TLC) and high performance liquid chromatographic (HPLC) methods established the presence of PGs in the extracts that were identified as PGA₂, PGB₂, PGD₂, POE₂, PGF₁α, PGF₂α, PGI₂ and non-identified forms of PGs, precursors or metabolites of PGs. The qualification/quantitative profiles for the PGs were very similar for adult, L3 and E/S products of T. colubriformis and H. contortus.