Determination of beta-agonists in biological samples by thin layer and liquid chromatographic methods

A simple and sensitive method for the determination of ß-agonists in animal tissue and urine using thin layer and liquid chromatography was prepared. The development of the method involves optimization of sample clean-up (solid-phase extraction) and chromatographic separation and detection conditions. The recovery of salbutamol and clenbuterol from tissue and urine samples were above 80% and the limit of detection was 1 ng g-1. The method has been used on a routine scale for residue control in samples from meat-producing animals.