Wykorzystanie lancuchowej reakcji polimerazy [PCR] do wykrywania i okreslania rozprzestrzeniania sie wirusa krwotocznego zapalenia jelit w organizmie indykow

The aim of the study was to evaluate the usefulness of the PCR method for detecting and investigating the distribution of haemorrhagic enteritis virus (HEV) in turkeys after oral inoculation. For the experiment forty-one day old turkey poults (But 9) were used. The birds were divided into two groups (20 birds in each group). The turkeys from the first group were orally challenged with a Polish HEV isolate at a dose of 10⁴˒³ EID₅₀, while turkeys from the second group were the unchallenged control group. Subsequently, three birds from each group were euthanized after 24, 48, 72, 96 and 120 hours of inoculation, and swabs were taken of spleen, kidneys, duodenum, cecal tonsils, bursa of Fabryci, heart, pancreas, thymus, brain, breast muscle, liver and gizzard during the autopsy. After conducting the PCR reaction with the starters HEV 1: 5’-TACTGCTGCTATTTGTTGTG-3’ and HEV2: 5’-TCATTAACTCCAGCAATTGG-3’ the final amplification product made of 1647 bp was received. The HEV was detected after 96 hours of inoculation in the duodenum, cecal tonsils, bursa of Fabryci, and in the spleen. 120 h after inoculation, the viral DNA not only was found in the same organs but also in the breast muscle and in the liver. The viral DNA was not detected after 24 and 72 h of the inoculation in the examined organs and after 96 and 120 h of inoculation in the kidneys, pancreas and in the gizzard. The results of the study show that PCR method enables an earlier detection of HEV in infected turkeys than the AGP test and confirms its usefulness for detecting infections caused by this virus.