Isolation and antimitotic activity of polysaccharides from fruit bodies of Xerocomus badius [Fr.] Kuhn. ex Gilib.

Two polysaccharide fractions were isolated from Xerocomus badius fruit bodies with a dry weight yield of 0.41% (Polysaccharide fraction A) and 0.89% (Polysaccharide fraction B). Their sugar content was 99.5% and 98.7%, respectively, Polysaccharide fraction A was composed of glucose, while Polysaccharide fraction B contained mainly glucose and mannose. Biological activity testing using the Allium test showed mitostatic and mitodepressant activity for both fractions. Polysaccharide fraction A exhibited a mitostatic action at a concentration of 0.5%, while Polysaccharide fraction B showed such activity at 0.5% and 0.25% concentrations after 24 h and 48 h. Polysaccharide fraction B exerted a direct influence on cell division of the tested organism in the Tetrahymena test.