Electrophoretic seed globulin patterns and species relationships in Vicia section Faba [Fabaceae]

This work presents electrophoretic seed globulin data obtained for 173 accessions representing nine Vicia species of the section Faba, which were already investigated for electrophoretic seed albumin patterns (PRZYBYLSKA, ZIMNIAK-PRZYBYLSKA, in press). Electrophoretic analysis of seed globulins was performed using sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE). In the examined material totally 38 well-defined polypeptide bands, in the relative molecular mass range of 19-61 kDa, were distinguished. The presence/absence of particular bands was recorded for each analysed individual. The electrophoretic data were used for hierarchical grouping of the examined taxa, based on calculated Bhattacharyya distances. V. bithynica proved to be the most distinctive member of Vicia section Faba. In turn, V. faba was clearly different from species of the V. narbonensis complex. Taxa of this complex formed two clusters: one consisting of V. narbonensis varieties and another - of the remaining species. In the latter group, V. serratifolia appeared to be rather distantly related to V. eristalioides, V. galilaea, V. hyaeniscyamus, V. Johannis and V. kalakhensis. The obtained results are compared with the corresponding seed albumin data and discussed with reference to taxonomie relationships in Vicia section Faba.