Use of the ELISA for determination of IgG anti-Pasteurella multocida serum level in geese vaccinated against pasteurellosis

The ELISA was used to measure anti-Pasteurella multocida IgG levels in the serum of 30 geese collected randomly from a 300-goose flock aged 2 months and vaccinated against pasteurellosis with the Pastivac E vaccine comprising Polish isolates of serotypes 1 and 3 with oil adjuvant. The blood was sampled every 14 days for 2 months from two groups of 10 birds each vaccinated singly and doubly and from non-vaccinated considered the controls. The ELISA kits were prepared by the author's procedure because no commercial kits for goose blood were available. An increase in anti-P. multocida IgG level was found after each vaccination.