Efekt kokultywacji komorek nerki owcy limfocytami krwi krowy bialaczkowej

Foetal lamb cells cocultivated with leukocytes of leukaemic cattle were passaged in monolayer from 2 to 35 passages. The degeneration signs in the cytoplasm and nucleus of the cells were observed following this cycle of multiplication. Generally, 43 passages of these cultures were conducted. The presence of the bovine leukaemia virus (BLV) in selected passages of these cells was confirmed by the syncytial test, PCR reaction and by electron microscopy. The presence of the virus structural proteins in the supernatant of the cell culture was confirmed by an agar gel immunodiffision test and by the immunoblot method.