Wplyw alkoholu etylowego i kofeiny na metabolizm wybranych rozpuszczalnikow organicznych w warunkach ekspozycji inhalacyjnej. Cz.X. Opracowanie koncowe ze szczegolnym uwzglednieniem wplywu alkoholu etylowego i kofeiny...

Oceniono wpływ alkoholu etylowego i kofeiny na wydalanie fenolu, kwasu benzoesowego i kwasu metylobenzoesowego - metabolitów stanowiących podstawę testów wykorzystywanych w biologiczym monitoringu narażenia na benzen, toluen i ksylen w warunkach przemysłowych.

This study was undertaken to investigate the influence of ethyl alcohol and caffeine on the biological monitoring parameters of exposure to benzene, toluene, and xylene. Experiments were conducted on 1584 adult male Wistar rats treated in a dynamic inhalation chamber with benzene, toluene, and xylene (separately or in combination) at different concentration (1.5, 4.5, or 13.5 mmol per m3 of air), for four consecutive days, 6 hours per day (from 8 a.m. till 2 p.m). Thirty minutes prior to every single experiment, rats were pretreated by stomach tube either with ethanol (aqueous solution) in a dose of 1366 or 2732 mg/kg body weight (1/10 ot 1/5 LD50) or with caffeine (aqueous solution) in a dose of 20 or 100 mg/kg body weight (1/10 or 1/2 LD50). The controls received distilled water only. Urine elimination of phenol, benzoic acid, and methylbenzoic acid (biological exposure indices) was measured within the first and the second day after termination of the exposure to solvents vapours, using gas chromatographic method. No effect of both ethanol and caffeine on urine elimination of selected biological indices was found in animals exposed to solvent vapours at the concentration of 1.5 mmol/m3 of air. In animals exposed to solvents vapours at the concentration of 4.5 mmol/m3 the excretion of compounds under test was found to be inhibited both by ethanol and caffeine. On the other hand, when animals were exposed to solvent vapours at the concentration of 13.5 mmol/m3, ethanol was founf to increase, but caffeine - to decrease urine elimination of phenol, benzoic acid, and methylbenzoic acid. These results indicate that ethanol and caffeine are important factors which may make difficult the interpretation of biological monitoring parameters in case of single or mixed exposure to benzene, toluene, and xylene at high concentrations.