Activity of lysosomal enzymes in acute intoxication with organophosphorus insecticides

The aim of this paper was to examine the activity of lysosomal enzymes after intoxication with chlorfenvinvos and dichlorvos - phosphoorganic insecticides. Investigations were carried out on male Wistar rats weighing 180-230 g. Rats were divided into two groups: control and treated. Rats of treated group were receiving oil solution of chlorfenvinvos at doses of 0.5 LD50 or 0.1 LD5(), determinations were performed after 1 and 24 hours after treatment or dichlorvos in the dose equal to 0.5 LD50 estimations were performed after 1 h only. Rats of control group were given equivalent volume of oil. Activities of beta-glucuronidase and acid phosphatase in the blood serum and lysosomal fraction of rat liver homogenate were determined. It was found that the level of ChE inhibition and increase of BGR activity in the serum were dependant on chemical structure of used compound, dose and time after treatment. It was observed that increase of hydrolases activities in serum was accompanied by the decrease in its activity in the lysosomal fraction of liver homogenate.