Application of 19 microsatellite DNA markers for parentage control in Borzoi dogs

The aim of this study was to analyse the polymorphism of 19 microsatellite markers and their usefulness for parentage verification in 28 Borzoi dogs in Poland. The assay involved the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and amelogenin. These markers are included in the panel of loci recommended by the ISAG. Amplified PCR products were analysed by an automated sequencer ABI PRISM 3130xl Genetic Analyser. The number of alleles per locus varied from 3 (INU005) to 8 (REN162 and AHT171). Except the INU005 locus, which showed the lowest variation (PIC=0.396, H=0.438), the calculated PIC values exceeded 0.6 and H values ranged from 0.677 (INU055) to as much as 0.84 (REN162). Based on PEC it was found that incorrect pedigree in this breed can be excluded with 99.9998% accuracy using DNA analysis.