Tytuł pozycji:
Zastosowanie odczynu immunofluorescencyjnego do identyfikacji parwowirusa psów (CPV 2) izolowanego w hodowli komórek FC
- Tytuł:
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Zastosowanie odczynu immunofluorescencyjnego do identyfikacji parwowirusa psów (CPV 2) izolowanego w hodowli komórek FC
Immunofluorescent test for the identification of canine parvovirus (PCV-2) propagated in a continuous FC line of cells
- Autorzy:
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Hulas C.
- Język:
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polski
- Dostawca treści:
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AGRO
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Przejdź do źródła Link otwiera się w nowym oknie
The samples of 300 faeces derived from the dogs suspected of canine parvovirosis were tested by the haemagglutination and haemagglutination-inhibition tests according to Carmichael. Out of the samples which had a titre of 4096 ten samples were taken at random to propagate CPV-2 in a continuous FC line of cells. After 72 hours the inoculated cells were examined by the direct immunofluorescent test using a labelled antibody against feline panleukopenia virus. A specific immunofluorescence was detected in cells inoculated with these samples. The specificity of the test was confirmed by blocking of the IF test by means of antibodies against CPV-2 virus.
