Physico-chemical characterization and microbial studies of muscle lipid of Liner silver grunter (Pomadasys hasta) of the Bay of Bengal

Lipid was extracted from the lipid containing muscle of Liner silver grunter (Pomadasys hasta) by solvent extraction and then purified by standard method. The physico-chemical properties of lipid sample were determined and compared with those of other standard fats and oils. Fatty acid composition of the lipid sample was investigated by Thin Layer Chromatographic (TLC) examination. Gas Liquid Chromatographic (GLC) examination was performed by the methyl esters mixture prepared from the lipid sample. The de-oiled muscle of the fish was studied for the determination of ash content, fiber content, moisture content and protein content. The lipid containing selected muscle was analyzed quantitatively for the determination of percentages of protein, cholesterol and minerals (N, P, K, Ca, and Na). The lipid containing muscle was also analyzed for the determination of the amount of eleven metals by using Atomic Absorption Spectrophotometric method. The lipid sample was evaluated by microbial activity (bacterial activity against nine bacteria and fungal activity against seven fungi).