Detekcja Phytophthora citrophthora w sztucznie zakażonych liściach przy wykorzystaniu techniki real-time PCR

Phytophthora species are considerable threat to the nurseries of trees, bushes and perennials. In Poland, several Phytophthora species were isolated from diseased plant, soil and water. P. citrophthora was isolated from deciduous and coniferous plants. With the use of real-time PCR technique with SYBR® Green and Pc2B/Pc primers and we were able to detect P. citrophthora in artificially infected rhododendron leaves with symptoms of colonization and in symptomless.