Immunohisto- and cytochemical localization of PR-10 proteins induced by heavy metals in lupine roots

Most of the data concerning PR-10 proteins accumulation in plants under various environmental stress conditions are derived from biochemical analysis of transcript expression or polypeptide accumulation. In the current study, we examined the localization of PR (L1–L6) proteins, belonging to the PR-10 class family, in the root tips of yellow lupine seedlings using histo- and cytochemical methods. Immunohistochemical localization revealed the presence of PR-proteins in all examined yellow lupine root tip tissue, and lead treatment did not change the distribution of proteins in the roots. However, the metal induced a significant increase in the accumulation of PR-proteins in the meristematic cells and procambium tissue. An immunocytochemical approach with immunogold method demonstrated the cytoplasmic nature of the PR-proteins and their increased accumulation after copper treatment. The results confirmed the affiliation of PR (L1– L6) to PR-10 class family proteins, as well as their intracellular localization.

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