The Effect of Extraction Process on The Antioxidant Activity of Prunus Serrotina L. Leaves Extracts

The study evaluated the antioxidant potential of Prunus serotina L. leaves extracts obtained with the use of purified water in the Mowo Aqua system, prepared in 5, 10 and 15 minutes. It was found that the purification system affects the properties of the obtained extracts. First of all, the osmolality of the extracts increased, which indicates the amount of extracted compounds. Water purification in the tested system resulted in an increase in the content of compounds reacting with the Folin Ciocalteu reagent. Their highest content was found in extracts prepared within 10 and 15 minutes. Higher content of polyphenols was responsible for higher antiradical activity in tests with DPPH radicals (5.41mg TE/1g d.m.) and ABTS cation radical (8.69 mg TE/1g d.m.). High antioxidant activity was also confirmed in the FRAP test, where extracts prepared with Mowo Aqua water showed up to 27% higher activity compared to control extracts. The above results were confirmed by the electrochemical method.