Imaging of retinal ganglion cells and photoreceptors using Spatio-Temporal Optical Coherence Tomography (STOC-T) without hardware-based adaptive optics

We demonstrate an experimental Spatio-Temporal Optical Coherence Tomography (STOC-T) system featuring optimized illumination and an increased lateral resolution of approximately 3 µm. The integration of high-speed phase randomization with a numerical averaging process facilitates a noticeable improvement in the signal-tonoise ratio. The effectiveness of this enhancement is demonstrated through volumetric imaging of a scattering object, and it enables in vivo imaging of the human retina at the cellular level. Additionally, the experiment is supported by computational aberration-correction techniques to achieve high-resolution in vivo imaging of the human retina. The visualization of retinal cone mosaics, and ganglion cell somas was achieved through contrast enhancement during the averaging process.

---

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025).