High-performance liquid chromatography separation of glycerol derivatives on a chiral stationary phase

We report herein the separation of enantiomers of glycerol derivatives on a cellulose-modified chiral column (Chiracel OD) in normal-phase mode. This method is suitable for monitoring the progress of the reaction and for determination of the enantiomeric purity of products. The lipase Novozyme 435-catalysed enantioselective hydrolysis of 1-O-benzyl-2,3-di-O-octanoyl-rac-glycerol has been shown to be an efficient method for preparation of 1-O-benzyl-2-O-octanoyl-sn-glycerol with high enantiomeric excess (ee > 95%).