High-performance liquid chromatographic determination of bergenin in different Bergenia species

Because previous chemical investigations have revealed the presence of bergenin and gallic acid as important bioactive components of Bergenia, a simple, highly precise RP-HPLC method coupled with photodiode-array detection has been developed and validated for simultaneous determination of these compounds in the Bergenia species B. ligulata (Wall) Eng., B. ciliata (Royle) Raizada, and B. stracheyi Engl. B. ciliata and B. stracheyi were found to contain the most bergenin, 3.275 and 3.277%, respectively; B. ligulata contained 2.419% bergenin.