Time-course data of the phosphorylation of MAP kinases in yeast cells treated with alpha-factor and/or salt

This dataset contains the results of an experiment meant to investigate crosstalk between Ste11-dependent mitogen-activated protein kinase (MAPK) pathways in cells of the yeast Saccharomyces cerevisiae. Yeast cells were stimulated with the hormone alpha-factor and with salt (NaCl) in three different scenarios: (1) only alpha-factor, (2) alpha-factor and salt simultaneously, (3) only salt. Samples were drawn from the cultures at defined time points after stimulation, total proteins were isolated and analyzed by Western blotting with antibodies against the phosphorylated forms of three yeast MAPKs: Fus3, Kss1 and Hog1. The Western blots were scanned and quantified using an Odyssey infrared scanner. This dataset contains raw quantitative phosphorylation data, processed data (including graphs), and scans of the blots.